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  • Product Name: Aflatoxin B1 Immunoaffinity Column
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Product Name: Aflatoxins B1 Immunoaffinity Column

Catalog No.: HCM0325/HCM0350A

Specification: 1mL 25/50T

Storage Condition: 2-8

Restriction: For research use only!

Intended Use

Total Aflatoxins B1Immunoaffinity Columnwith HPLCis a quantitative method for the detection of Aflatoxin B1 in samples like gains, nuts, feed, etc. Samples are purified by the Column before quantitated by high performance liquid chromatography (HPLC).

Immunoaffinity Column with HPLC can improve signal-noise ratio (SNR) and increase the accuracy of the detection results。

Summary

Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is considered the most toxic. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

Principle

The detection is based in antigen-antibody reaction. The antibody is bound in the column.

Samples are prepared by mixing with an extraction solution, blending and filtering。 The extract is then applied to the column bound with specific antibodies to aflatoxin B1。 At this stage, the aflatoxin B1 bind to the antibody on the column。 The column is then washed to rid the immunoaffinity column of impurities。 By passing methanol through the column, the aflatoxins are removed from the antibody。 This methanol solution can then be injected into an HPLC system。

Kit Composition

Each kit contains aflatoxins B1 Immunoaffinity Columns of different specifications and one copy of Instruction for Use。

Capacity: 300ng

Recovery: 90%-110%

Specificity: High specificity for aflatoxins

Flow Rate: Almost 100%, control the flow speed at about 1 drop/sec.

Aflatoxins B1 Immunoaffinity Column Assay Procedure

 

  1. Take out the column and remove the plug on the top; install the adapter and connect the other end of the adapter to the syringe (for 3ml column use only)。
  2. Fix the column with syringe on the Air-Pressure Controller。
  3. Remove the plug on the bottom of the column and pass the filtered diluted extract completely through the immunoaffinity column at a rate of about 1-2 drops/second until air comes through column.
  4. Pass 10 ml of distilled water through the column at a rate of about 2-3 drops/second。
  5. Remove the syringe from the column, place a test tube under the column and pass 1ml methanol at a rate of 1 drop/second.
  6. Mix well and filter through 0。22μm syringe filter to a sample bottle。 Inject the dilute onto HPLC。

* The immunoaffinity column should be brought to room temperature, 22 – 25ºC prior to use。

* Drain the liquid in the column of last operation before start a new injection.

* This method is also applicable with GB/T 18979-2003 and SNT 1736-2006.

Result Interpretation

The content of aflatoxins = Detected concentration × Dilution factor

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