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  • Product Name: Melamine ELISA Test Kit
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Product Name: Melamine (MEL) ELISA Kit

Catalog No.: HE09037/HE12003

Specification: 96T/48T

Storage Condition: 2-8

Restriction: For research use only!

Summary

Melamine is described as "Harmful if swallowed, inhaled or absorbed through the skin. Chronic exposure may cause cancer or reproductive damage. Eye, skin and respiratory irritant." However, the short-term lethal dose is on a par with common table salt with an LD50 of more than 3 grams per kilogram of bodyweight.

Use Principles

Melamine ELISA Kitis an indirect competitive enzyme-labeled immunoassay. The tetracycline antigen is precoated on the wells. The precoated antigen compete the tetracycline antibody (antibody solution) with tetracycline in the sample, anti- tetracycline antibody binds to the tetracycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color. 

The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived。

Scope of Application

Qualitative or quantitative analysis the residues of Melamine in raw milk, milk powder, yoghourt, egg, feed, etc.

Cross-reactivity

Melamine…………………………………………………100%

Provided Materials and Reagent

Component

96 wells/48wells

Antigen coated plate

12 test strips of 8 wells each/6 test strips of 8 wells each

6 vials of standard

1ml each/0.5ml each

High concentration standard 1ppm

1ml/0.5ml

HRP-conjugate

6ml/3ml

Antibody Solution

6ml/3ml

Substrate A

6ml/3ml

Substrate B

6ml/3ml

Stop Solution

6ml/3ml

Concentrated Sample redissolving solution 16X

12ml/6ml

Concentrated Wash Buffer (10×)

40ml X2/20mlX2

IFU

1

Testing Report

1

Sensitivity, accuracy and precision

Sensitivity:            1ppb

Limit of detection

Milk powder……………………………………………………………………………………50ppb

Yoghourt….……………………………………………………………………………………10ppb

Fresh Milk………………………………………………………………………………………50ppb

Feed…。…………………………………………………………………………………………50ppb

Egg…。…………………………………………………………………………………………。10ppb

Percent Recovery, %

Milk powder…………………………………………………………………………………100±20%

Yoghourt….…………………………………………………………………………………100±20%

Fresh Milk……………………………………………………………………………………100±20%

Feed….………………………………………………………………………………………100±20%

Egg….………………………………………………………………………………………..100±20%

Precision (CV %):

Intra-lab assay: CV%≤10%

Melamine (MEL) ELISA Kit Assay Procedure

 

READ INSTRUCTION MUNUAL COMPLETELY BEFORE USE!

1.  Take out the reagents from the refrigerator and allow them to reach room temperature (2025) prior to running the test. Shake up the reagents before use.

2.  Place the appropriate number of test wells into a microwell holder. Be sure to re-seal unused wells in the zip-lock bag with desiccant stored under the temperature 2-8(Do not freeze)。

3.  Numbering: Number the microwells for the standards and samples separately. Running standards and samples in duplicate.

4.  Using a pipette with disposable tips, add 50 μl of standards and samples to the appropriate test wells. Be sure to use a clean pipet tip for each. Then dispense 50µl of HRP-conjugate and 50µl of antibody solution into each test well in order.

5.  Shake the plate gently to mix contents, cover the plate rack and incubate the test wells at 25keeping away from light for 30 minutes.

6.  Open the cover on the plate and dump the contents of the wells into an appropriate waste container. Fill the wells to overflowing with wash buffer and dump (300µL each well). Repeat for a total of five washes (wait 10s between each wash). Following the last wash, tap the inverted wells onto absorbent paper to remove the last of the wash buffer.

7。  Dispense 50µL Substrate A and 50µL Substrate B into each well in order。 Shake the plate gently。 Cover the plate rack and incubate the wells at 25for 15 minutes.

8.  Dispense 50 µL of stop solution into each test well. Shake the plate rack gently to mix.

9.  Read and record the absorbance of the wells at 450nm using a plate reader (dual wavelength 450/630nm detecting is advised) within 5 minutes.

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